Titlebook: Long Non-Coding RNAs; Methods and Protocol Lin Zhang,Xiaowen Hu Book 2021Latest edition The Editor(s) (if applicable) and The Author(s), un

驚惶

Assessment of In Vivo siRNA Delivery in Cancer Mouse Models,he administration site to target cells in vivo. This chapter describes approaches to deliver siRNA effectively for cancer treatment and discusses in detail the current methods to assess pharmacokinetics and biodistribution of siRNAs in vivo.

TAIN

Characterization of Circular RNAs,ired specific treatments for their identification and validation. Here, we describe a detailed pipeline for the characterization of circular RNAs. It has been successfully applied to the study of circular intronic RNAs (ciRNAs) derived from intron lariats and circular RNAs (circRNAs) produced from back spliced exons in human.

不如樂(lè)死去

Consequence

Chandelier

Book 2021Latest editionRNA genes to molecular and cellular analyses of the function of individual lncRNA.?Chapters guide readers through studies used to circular RNAs, RNA splicing, and findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include intro

指耕作

Defiance

Characterization of Long Non-coding RNA Associated Proteins by RNA-Immunoprecipitation,roteins with specific RNA molecules in vivo. It can be used to investigate lncRNA–protein interaction and identify lncRNAs that bind to a protein of interest. Here we describe the protocol of this assay with detailed materials and methods.

HAIL

單調(diào)性

Capturing Endogenous Long Noncoding RNAs and Their Binding Proteins Using Chromatin Isolation by RNs, such as artifacts caused by tagging or non-physiological interactions. Here we describe the detailed protocol for chromatin isolation by RNA purification (ChIRP) from mammalian cell lines and mouse tissues, which captures endogenous lncRNAs and enables subsequent identification of their physiologically relevant binding partners.

擴(kuò)張

Detection of Long Non-coding RNA Expression by Non-radioactive Northern Blots,re various. Northern blot analysis can help us learn about the identity, size, and abundance of lncRNAs. Here we describe how to use northern blot to determine lncRNA abundance and identify different splicing variants of a given lncRNA