Titlebook: Listeria Monocytogenes; Methods and Protocol Edward M. Fox,Hélène Bierne,Beatrix Stessl Book 2021Latest edition Springer Science+Business M

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Traditional Methods of Analysis for Conventional methods for the detection of . in foods and environmental samples rely on selective pre-enrichment, enrichment, and plating. This is followed by confirmation of suspected colonies by testing a limited number of biochemical markers.

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Multilocus Sequence Typing (MLST) and Whole Genome Sequencing (WGS) of , and d in the core genome (core genome MLST) facilitate the rapid and interlaboratory comparison in open accessible databases as provided by Institute Pasteur (.). Strains can be compared on a global level and help to track forward and trace backward pathogen contamination events in food processing facilities and in outbreak scenarios.

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Generation of Nonpolar Deletion Mutants in , Using the “SOEing” Methoddepth a protocol for the creation of nonpolar unlabelled gene deletions in . that are facilitated by the splicing overlap extension PCR technique. For mutagenesis in . we describe the pKSV7 plasmid-based approach, which facilitates the introduction of a spliced amplicon in place of the corresponding segment of chromosomal DNA.