Titlebook: Lipid Signalling; Methods and Protocol Marco Brotto,Sanjoy K. Bhattacharya Book 2024 The Editor(s) (if applicable) and The Author(s), under

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1064-3745 ng known pitfalls...Authoritative and comprehensive, .Lipid Signaling: Methods and Protocols. is a valuable resource for researchers looking to expand their knowledge of this important and developing field..978-1-0716-3904-7978-1-0716-3902-3Series ISSN 1064-3745 Series E-ISSN 1940-6029

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978-1-0716-3904-7The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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https://doi.org/10.1007/978-1-0716-3902-3arachidonic acid; Systems Biology; Spatial Restriction; Stereochemistry; Enzymes

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Measurement of Intracellular Ca2+ for Studying GPCR-Mediated Lipid Signaling,can be studied by intracellular Ca. imaging. Here we describe the protocols to measure intracellular Ca. for studying PEG2-EP1 activity in esophageal smooth muscle cells. The ratiometric Fura-2 imaging provides quantitative data, and the Fluo-4 confocal microscopic imaging has high-spatial resolution.

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Marco Brotto,Sanjoy K. BhattacharyaIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Method to Assess the Intracellular Fate and Bioavailability of Clusterin Using Live Cell Confocal Mell understood. The protocol described here utilizes clusterin tagged to fluorescent proteins like green fluorescent protein and red fluorescent protein to understand the intracellular fate of clusterin.

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