Titlebook: Ionotropic Glutamate Receptor Technologies; Gabriela K. Popescu Book 2016 Springer Science+Business Media New York 2016 atomic organizatio

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Assaying the Energetics of NMDA Receptor Pore Openingges in synaptic connectivity and strength. A variety of approaches are used to address the linkage between neurotransmitter binding and ion channel opening, a key feature common to all ligand-gated ion channels. Φ-value or REFER analysis, an approach derived from chemical transition state theory, ca

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Assessing The Effects of Ligand-Binding Mutations to AMPA and Kainate Receptor Kineticstiate a sequence of conformational steps that lead to channel opening. In the case of AMPA and KA receptors, they also lead to very rapid desensitization on a comparable time scale. Precisely which residues are most important to which processes, and how the receptor–ligand interactions impact variou

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Analysis of Whole-Cell NMDA Receptor Currentsation. The probability of finding the receptor in a particular conformation is given by the apparent rate constants. The apparent rate constants describe the rate of transitions between conformational states and vary among different types of iGluR. To determine these parameters reflecting receptor c

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Gabriela K. PopescuIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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