Titlebook: Intrinsically Disordered Protein Analysis; Volume 1, Methods a Vladimir N. Uversky,A. Keith Dunker Book 2012 Springer Science+Business Med

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Determination of IUP Based on Susceptibility for Degradation by Defaultchanisms, of which a more general one is interaction with a partner named nanny. Based on these attributes one can define IUP by conducting a set of cell free and cell culture experiments as outlined in this chapter.

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In-Cell NMR of Intrinsically Disordered Proteins in Prokaryotic Cellsn-cell NMR, particularly employing commonly used protein overexpression systems in . cells, is the most accessible approach. In this chapter we describe general procedures for obtaining in-cell NMR spectra in . cells.

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In-Cell NMR in Mammalian Cells: Part 2ple passive diffusion. Therefore, proteins that are to be delivered into mammalian cells are not required to contain additional “targeting” sequences, and can be employed in their native contexts. The protocol outlined here employs isotope-labeled human α-synuclein, adherent human HeLa cells, and the . endotoxin Streptolysin O (SLO).

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Fourier Transform Infrared Microspectroscopy of Complex Biological Systems: From Intact Cells to Whozed procedures to prepare biological samples for IR measurements and the method to collect and analyze their FTIR spectra. In particular, we describe here the investigations on bacterial cells, intact eukaryotic cells, and whole intact nematode specimens.

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Studying IDP Stability and Dynamics by Fast Relaxation Imaging in Living Cellsroteins near the structured–unstructured boundary are particularly sensitive to their environment. We describe, using the IDP α-synuclein as an example, how FReI can be used to measure IDP stability and folding inside the cell.

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1064-3745 sults.Contains key notes and implementation advice from the .Over the past decade, there has been an explosive development of research of intrinsically disordered proteins (IDPs), which are also known as unfolded proteins. Structural biologists now recognize that the functional diversity provided by

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