Titlebook: Intracellular Lipid Transport; Methods and Protocol Guillaume Drin Book 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Bi- and Trifunctional Lipids for Visualization of Sphingolipid Dynamics within the Cell,f living cells, since fluorescent modifications drastically change lipid properties. Here, we describe the use of bifunctional as well as trifunctional sphingosine to reveal its subcellular localization via crosslinking, fixation, and specific staining by click reaction with a fluorophore. Additiona

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Indirect Lipid Transfer Protein Activity Measurements Using Quantification of Glycosphingolipid Proe yet that directly would address the lipid transfer protein activity in vivo. Therefore, these approaches can serve as tools to indirectly study the lipid transfer protein activity in cells, by monitoring their impact on the glycosphingolipid homeostasis.

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Measurement of Intracellular Sterol Transport in Yeast,sfer it to another across the cytoplasm. Here we describe a suite of complementary assays to measure intracellular sterol transport in the model eukaryote ., as well as to quantify protein-mediated sterol transport between populations of vesicles in vitro. The in vivo assays can be adapted to study

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