Titlebook: Immunohistochemistry and Immunocytochemistry; Methods and Protocol Luis Del Valle Book 2022 Springer Science+Business Media, LLC, part of S

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Fluorescent In Situ Hybridization (FISH),rations. Both enzymatic and fluorescent ISH studies can be used as adjunct studies, and in some cases are vital to making specific diagnoses. This chapter will focus on fluorescent in situ hybridization.

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Culture and Phenotyping of Glial Cell Cultures, Gliospheres , and Neurospheres,rain-derived cells in monolayer presents significant challenges that obscure the fidelity of in vitro results. After a few number of passages, glial and neuronal cells begin to lose their morphological characteristics, and most importantly, their specific cellular markers and phenotype. In recent ye

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ER+ Breast Cancer Mammosphere Formation and Analysis, breast cancer stem cell (BCSC) contribution to tumorigenesis, as well as more closely mimicking the three-dimensional tumor microenvironment. In these assays, BCSCs are stimulated to proliferate in low adherence tissue culture dishes and the resulting mammospheres exhibit activation of stem cell-re

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Comet Assay for the Detection of Single and Double-Strand DNA Breaks,on, DNA damaging chemicals, and viral infections. Rapid, sensitive, and economical methods for the detection of DNA damage offer valuable insights into DNA repair, drug genotoxicity, and biomonitoring. The comet assay, or single cell electrophoresis, has emerged as a popular method for detecting sin

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Fluorescent-Activated Cell Sorting ( Flow Cytometry ),on of different hematolymphoid neoplasms (Reichard KK, KS, Flow cytometry in the assessment of hematologic disorders. In: Orazi A, Foucar K, Knowles DM et al (eds) Neoplastic hematopathology. Lippincott, Williams and Wilkins, Baltimore, MD, pp 119–145, 2013). Flow cytometry allows us to identify ind

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