Titlebook: Immunohistochemistry; Essential Elements a Alexander E. Kalyuzhny Book 2016 Springer International Publishing Switzerland 2016 electrophysi

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胡言亂語

Choosing Commercial Suppliers of Primary Antibodies,f primary antibodies, either generated in-house or purchased from commercial suppliers. It appears that by choosing the in-house route, researchers are in full control of the antibody production process and can generate an endless supply of antibodies free of charge that can be shared with other labs.

Amendment

Staining Protocols,iversal “one-size-fits-all” staining protocols in IHC: the approach that works for a particular type of tissue and “to-be detected” antigens may be completely ineffective for other types of tissue and antigens. That is why the most critical step in IHC is the optimization of a staining protocol allowing for the best detection of tissue antigens.

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978-3-319-80910-6Springer International Publishing Switzerland 2016

樹木中

Immunohistochemistry978-3-319-30893-7Series ISSN 2367-1114 Series E-ISSN 2367-1122

intellect

Short Historic Overview,Immunohistochemistry, or IHC, is a technique that utilizes antibodies—products of the immune system—to analyze histological tissues under the microscope. IHC evolved from histochemistry, a technique that utilizes chemical and biochemical techniques for probe histological tissues.

有惡意

Detection and Visualization of Antigens,As we know now, the first step in detecting tissue antigens requires incubation of tissue sections with the primary antibodies. However, this does not allow for the detection and visualization of the antigen-antibody complex because primary antibodies are colorless.

漂白

該得

ambivalence