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Titlebook: Immunocytochemical Methods and Protocols; Lorette C. Javois Book 19951st edition Humana Press 1995

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發(fā)表于 2025-3-21 17:03:32 | 只看該作者 |倒序瀏覽 |閱讀模式
書目名稱Immunocytochemical Methods and Protocols
編輯Lorette C. Javois
視頻videohttp://file.papertrans.cn/463/462125/462125.mp4
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: Immunocytochemical Methods and Protocols;  Lorette C. Javois Book 19951st edition Humana Press 1995
描述The principle that antibodies can be used as cytochemical agents provided they are tagged with suitable markers has been evident for over 50 years. During this time the use of immunocytochemical meth- ods has spread to a wide array of biological disciplines. Early applica- tions focused on the detection of microbial antigens in tissues, while more recent applications have used monoclonal antibodies to study cell differentiation during embryonic development. For a select few disci- plines, volumes have been published focusing on the specific applica- tion of immunocytochemical techniques to that discipline. What distinguishes the present book, Immunocytochemical Meth- ods and Protocols, from earlier books is its broad appeal to researchers in all disciplines, including those in both research and clinical settings. The methods and protocols presented here are designed to be general in their application and the accompanying "Notes" provide invaluable assistance in adapting or troubleshooting the protocols. Interspersed throughout the book are chapters providing overviews of select topics related to immunocytochemistry.
出版日期Book 19951st edition
版次1
doihttps://doi.org/10.1385/089603285X
isbn_ebook978-1-59259-521-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 1995
The information of publication is updating

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Purification of Antibodies Using Ammonium Sulfate Fractionation or Gel Filtration,um sulfate fractionation, is generally employed as the initial step in the isolation of crude antibodies from serum or ascitic fluid (.#x2013;.). Ammonium sulfate precipitation, in many instances, is still the method of choice because it offers a number of advantages. Ammonium sulfate fractionation
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Purification of Antibodies Using Affinity Chromatography,is type of separation, unlike other chromatographic methods, uses the intrinsic biological activity of a molecule to bind to a substrate, hapten, or antigen. Principles of matrix selection, gel preparation, and coupling of ligands have been reviewed extensively by Ostrove (.). Antibody affinity chro
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Conjugation of Fluorochromes to Antibodies,t first, the specific antibody itself was labeled and applied to the tissue section to identify the antigenic sites (direct method) (.). Later, the more sensitive and versatile indirect method (.) was introduced. The primary, unlabeled, antibody is applied to the tissue section, and the excess is wa
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Biotinylation of Antibodies,ty chromatography, and many other areas (.) (.. and .). It was first exploited in immunocytochemical applications in the mid-1970s (.,.), and has since been commonly used to localize antigens in cells and tissues. In this technique, a biotinylated primary or secondary antibody is first applied to th
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Processing of Cell-Touch Preparations,ing into them precludes the use of frozen sections. Touch preparations or imprints enable the examination of the whole cell apart from the tissue aspect. The cells are obtained by touching a wet tissue with a glass slide. Cells adhere to the glass in roughly the same orientation as they exist on the
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