Titlebook: Horizontal Gene Transfer; Methods and Protocol Fernando de la Cruz Book 2020 Springer Science+Business Media, LLC, part of Springer Nature

青少年

Quantifying and Characterizing Distributive Conjugal Transfer in the species. Mycobacteria utilize an atypical method of conjugation called distributive conjugal transfer (DCT), which results in mosaic genomes and the potential for accelerated evolution beyond that enabled by the more classical .-mediated conjugation. The following is a description of the basic D

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Spectrophotometric Assays to Quantify the Activity of T4SS ATPaseshe nucleoprotein complex to the recipient cell. In the conjugative plasmid R388, these ATPases are named TrwB, TrwK, and TrwD. Here, three different spectrophotometric assays to measure the enzymatic properties of these ATPases are described. The choice of the assay will depend on the specific requi

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Methods to Identify and Analyze Vesicle-Protected DNA TransferIn addition, alternative HGT mechanisms exist in groups of organisms. Among them, the use of DNA-containing membrane vesicles as shuttle elements for HGT has been described for a number of?microorganisms, including both thermophiles and mesophiles. Here we describe the methods followed to detect, pu

Halfhearted

Measuring Plasmid Stability in Gram-Negative Bacteriaselection of plasmid-containing cells using an . cassette. When bacteria carrying the . module in the plasmid of interest are grown in media containing rhamnose as the only carbon source, the . promoter is induced, ParE is synthesized, and plasmid-containing cells are eliminated; bacteria that have

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on, and control. The use of ML components is projected to grow and with it the concern of using these components in systems that operate in safety-critical settings. To guarantee a safe operation of autonomous systems, it is important to run an ML component in its operational design domain (ODD), i.