Titlebook: High-Speed Atomic Force Microscopy in Biology; Directly Watching Dy Toshio Ando Book 2022 Springer-Verlag GmbH Germany, part of Springer Na

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Cantilever MechanicsIn Sect.?., some mechanical properties of a cantilever beam are briefly described to explain AFM imaging modes. Since AFM relies on the mechanics of a cantilever beam as a sensor, this chapter focuses on its fundamental mechanics.

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Feedback Control and Imaging RateAchieving feedback control to maintain the tip–sample contact strength is essential in the AFM imaging of biological samples. However, feedback control with a chasing-after nature cannot be carried out in real time; there is always a time delay and hence a control error. Faster imaging requires faster feedback control with a shorter time delay.

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Peripheral Membrane Proteins (PMPs)PMPs play important roles in various biological processes, including cell signaling, membrane trafficking, metabolism of membrane components, transport of hydrophobic molecules, and the formation of membrane pores.

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1434-4904 ew scanning probe microscopy techniques.A valuable guide forThis first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Num

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Toward the Next Generation of HS-AFMS Nano 15:17613–17622, .; Watanabe et al. in Rev. Sci. Instrum. 90:123704, .;), although its spatiotemporal resolution is still far lower than that of HS-AFM. This chapter describes very recent progress in the basic performances of HS-AFM (i.e., higher imaging rate and lower disturbance), followed by future prospects of HS-AFM performances.