Titlebook: Herpes Simplex Virus Protocols; S. Moira Brown,Alasdair R. MacLean Book 1998 Humana Press 1998

百靈鳥

Ancillary

Expression of HSV Proteins in Bacteria,s of specific viral proteins for use in both biochemical and functional analysis, and as immunogens for antisera production. Proteins can be expressed either in the full-length native form or as fusion proteins with affinity tails.

Lymphocyte

MILL

Analysis of HSV-1 Transcripts by RNA-PCR,ure of many HSV-1 RNAs can be obtained without the considerable time and effort that is required to construct and analyze cDNA libraries. Once the 5′ and 3′ ends of an RNA have been mapped precisely, the RNA nucleotide sequence can be deduced simply from the genomic DNA sequence. However, there are

BILIO

lanugo

HSV Amplicons in Gene Therapy,s, such as fibroblasts, macrophages, glia, and neurons in different organisms including rodents, monkeys, and human (refs. .–.; reviewed in ref. .). The defective viruses follow their nondefective counterparts in the ability to infect mitotic, as well as postmitotic cells. This makes them potentiall

HARP

Lobotomy

HSV-Cellular Protein Interactions,diate-early (IE) genes, followed by the early genes, and finally late gene expression (.). Although such a temporal cascade of viral gene expression involves the action of virally encoded regulatory proteins, such factors act, at least in part, by interacting with cellular transcription factors that

仇恨

有害處