Titlebook: Hepatocytes; Methods and Protocol Naoki Tanimizu Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

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Application of EdU-Based DNA Synthesis Assay to Measure Hepatocyte Proliferation In Situ During Livules and pathways as well as the regulatory mechanisms involved in liver regeneration. Periodic measurement of the liver/body mass ratio estimates the rate of liver regeneration, which is often supplemented by evaluating the proportion of proliferating hepatocytes using a synthetic nucleoside analog

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Identification of Genes Regulating Hepatocyte Injury by a Genome-Wide CRISPR-Cas9 Screen,ene editing can be employed to study directly the functional impact of stable gene knockout, activation, and knockdown. Here, we describe the end-to-end methodology by which we employ genome-wide CRISPR-Cas9 knockout to study drug toxicity using acetaminophen (APAP) in a hepatocellular carcinoma liv