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Titlebook: HPLC of Peptides and Proteins; Methods and Protocol Marie-Isabel Aguilar Book 2004 Humana Press 2004

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書目名稱HPLC of Peptides and Proteins
副標(biāo)題Methods and Protocol
編輯Marie-Isabel Aguilar
視頻videohttp://file.papertrans.cn/421/420305/420305.mp4
概述Includes supplementary material:
叢書名稱Methods in Molecular Biology
圖書封面Titlebook: HPLC of Peptides and Proteins; Methods and Protocol Marie-Isabel Aguilar Book 2004 Humana Press 2004
描述The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only i
出版日期Book 2004
版次1
doihttps://doi.org/10.1385/1592597424
isbn_softcover978-1-61737-286-5
isbn_ebook978-1-59259-742-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightHumana Press 2004
The information of publication is updating

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Methods in Molecular Biologyhttp://image.papertrans.cn/h/image/420305.jpg
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HPLC of Peptides and Proteinsmolecules. In particular, HPLC in its various modes has become the central technique in the characterization of peptides and proteins and has, therefore, played a critical role in the rapid advances in the biological and biomedical sciences over the last 10 years.
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https://doi.org/10.1007/978-3-662-10343-2of peptides (or protein-derived peptides) in the mass spectrometer using the technique of collision-induced dissociation (CID). The fragments generated with CID all originate from the precursor; thus, supplementary information relating to the primary sequence and post-translational modifications of
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