Titlebook: Glycovirology Protocols; Richard J. Sugrue Book 2007 Humana Press 2007 Hepatitis.Polyacrylamid-Gelelektrophorese.antibody.biology.infectio

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Erratum zu: Leistungselektronik,e (GST)-tagged proteins. The sequence covering the F. subunit (GST-F2), and a truncated form of the F protein in which the transmembrane domain was removed (GST-F./F., were cloned into the baculovirus pAcSecG2T secretory vector. These virus sequences also had the endogenous virus signal sequence rem

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WS-Umrichter, Wechselstromsteller,ssified into six major genotypes that exhibit extensive genetic variability, particularly in the envelope glycoproteins E1 and E2. Knowledge of genotypic and quasispecies variation on viral glycoprotein properties is important in understanding the structure-function relationship of the proteins. Thr

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Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate,AGs are sulfated to varying extents, lending a negative charge, and are used by a large number of viruses to initiate infection of immortalized cell lines. Here we describe the rationale and methods for analyzing GAG usage by one such virus, respiratory syncytial virus (RS V). The protocols presente

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Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coroe (SARS)-coronavirus (CoV) 3a protein. Using the same expression system, two structural proteins, membrane (M) and envelope (E), were co-expressed to form SARS-CoV virus-like particles (VLPs) within an insect cell. Expression of viral proteins was confirmed by Western blot analysis and the formation

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The C Type Lectins DC-SIGN and L-SIGN,eral enveloped viruses. DC-SIGN and L-SIGN enhance viral entry and facilitate infection of cells that express the cognate entry receptor (.-infection). They are also able to capture viruses and transfer viral infections to other target cells (.-infection). In this chapter, we will give an overview o

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Fibrillation

Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functionaexpressed viral glycoproteins are described. These methods are based on many years of experience with the influenza hemagglutinin glycoprotein (HA). However, they are applicable for studies on other viral glycoproteins, and with slight modifications, could be useful for cellular proteins as well.

gene-therapy

The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytia cleavage to give the disulphide bond-linked F1 and F2 subunits. The methodology detailing the use of two-dimensional electrophoresis, endoglycosidases, and α-mannosidase inhibitors, as applied to investigating F protein glycan maturation, is given. Examples are used to show how this methodology was