Titlebook: Germline Stem Cells; Michael Buszczak Book 2017Latest edition Springer Science+Business Media New York 2017 Germline stem cell.primordial

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Extensions of Rings and Modulesme integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.

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Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies,me integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.

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