Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2023Latest edition The Editor(s) (if applicable) and The

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Construction of Synthetic VHH Libraries in Ribosome Display Format,e system..Recently, synthetic VHH libraries have been designed to avoid the use of animals. Here, we describe the construction of VHH combinatorial libraries and their use for the selection of binders by ribosome display, a fully in vitro selection technique.

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Affinity Maturation of the Natural Ligand (B7-H6) for Natural Cytotoxicity Receptor NKp30 by Yeast s toward their cognate receptor, potentially hampering killing capacities of immunoligands. Herein, we provide protocols for yeast surface display-based affinity maturation of B7-H6, the natural ligand of NK cell-activating receptor NKp30.

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,Erw?rmung der elektrischen Leiter,ng strategy to identify protein variants with a stable transient binding pocket with improved binding for a cryptic site-specific ligand. This strategy may facilitate drug discovery using the resulting protein variants with accessible binding pockets for ligand screening.

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https://doi.org/10.1007/978-3-642-91141-5ional mammalian expression vector. Herein, we describe in-depth protocols for the reformatting of mAbs, starting from the generation of Fab fragment libraries in YSD vectors and ending up with IgG molecules in bidirectional mammalian vectors in a consolidated two-pot, two-step procedure.

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A Two-Step Golden Gate Cloning Procedure for the Generation of Natively Paired YSD Fab Libraries,lity and versatility of in vitro antibody display with the advantages of natively paired VH–VL antibodies. In this regard, VH–VL amplicons are cloned via a two-step Golden Gate cloning procedure, allowing the display of Fab fragments on yeast cells.