Titlebook: Genomics of Cereal Crops; Shabir Hussain Wani,Anuj Kumar Book 2022 The Editor(s) (if applicable) and The Author(s), under exclusive licens

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Check CRISPR Editing Events in Transgenic Wheat with Next-Generation Sequencing,GS) is the best choice for screening CRISPR-Cas9 induced edits. With NGS, hundreds to thousands of T1 plants can be sequenced without genome-specific primers. Here we present the detailed procedure for using NGS to select plants with the best editing, including primer design, PCR setup, sample preparation, and sequencing result analysis.

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Alireza Bahadori,Scott T. Smithhanges, and increase in population, hence calling for the utilization of innovative technologies to overcome the constraints of crop production. Crop improvement through multifaceted approaches that combine conventional and genomic technologies is necessary for developing biotic and abiotic stress-t

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https://doi.org/10.1007/978-1-4419-9713-5vents. To select the best plants for future study, these plants need to be screened for editing type and proportion. However, the traditional restriction enzyme method cannot tell the editing type and Sanger sequencing cannot check a mixture of different editing events. Next-generation sequencing (N

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https://doi.org/10.1007/978-1-4615-6838-4 reference genome of wheat (IWGSC RefSeq v2.1.) but also in pangenomic sequencing of cultivars of different origin including stress tolerant and susceptible cultivars, and transcriptomic sequencing of lines resistant/tolerant to different biotic and abiotic stresses. However, despite the vast progre

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Dictionary of Foreign QuotationsNA in a variety of biological processes. RNA characteristics have been investigated in a number of researches employing whole transcriptome sequencing (RNA-seq) data in all the major crops. To date, a number of crop genomes have been sequenced, which allows researchers to understand the typical biol