Titlebook: Genome Editing in Animals; Methods and Protocol Izuho Hatada Book 2017 Springer Science+Business Media LLC 2017 enzymes.TALEN.ZFN.CRISPR.ze

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書目名稱Genome Editing in Animals影響因子(影響力)




書目名稱Genome Editing in Animals影響因子(影響力)學(xué)科排名




書目名稱Genome Editing in Animals網(wǎng)絡(luò)公開度




書目名稱Genome Editing in Animals網(wǎng)絡(luò)公開度學(xué)科排名




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書目名稱Genome Editing in Animals被引頻次學(xué)科排名




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書目名稱Genome Editing in Animals年度引用學(xué)科排名




書目名稱Genome Editing in Animals讀者反饋




書目名稱Genome Editing in Animals讀者反饋學(xué)科排名

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https://doi.org/10.1007/978-1-4615-9723-0 results revealed that at least five gRNA sequences, each of them having only one perfectly complementary site in the whole genome, could be designed for more than 95% of genes, regardless of the organism. Next, among those gRNAs, we selected gRNAs that did not have any other complementary site to t

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Stakeholders, Programmes and Strategies,mizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, var

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https://doi.org/10.1007/978-1-4615-9723-0the CRISPR/Cas system, the RNA-guided endonuclease Cas protein introduces a DNA double-stranded break at the genome position recognized by a guide RNA (gRNA) based on complementary base-pairing of about 20-nucleotides in length. The 8- or 12-mer gRNA sequence in the proximal region is especially imp