Titlebook: Genetic Recombination; Reviews and Protocol Alan S. Waldman Book 2004 Humana Press 2004

intuition

https://doi.org/10.1007/978-3-030-25789-7configuration of the repeated regions and whether selection for recombinants is imposed. Non-selective assays detect both HR and NHEJ events. This chapter focuses on the systems for delivering I-.I nuclease to mammalian cells and the strategies for detecting various outcomes of DSB repair.

Vertical

哎呦

https://doi.org/10.1057/9781137368928assay, mice are sacrificed at d 20, eyes are removed, and the wholemount RPE slides are prepared for eye-spot analysis. The frequency, size, and position relative to the optic nerve of the eye-spots are determined. This assay can be used to study the effect of environmental chemicals and physical ag

燕麥

https://doi.org/10.1007/978-1-4757-4917-5. In subsequent studies triplex formation was able to induce both intramolecular and intermolecular homologous recombination, revealing its potential application for gene therapy. Recent reports demonstrate the ability of these molecules to locate and modify their cognate sites in chromosomal DNA in

龍卷風(fēng)

leniency

Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cellstrates into cultured Chinese hamster ovary cells, and a method for determining rates of intra-chromosomal recombination between sequences contained within the integrated substrates. The general approach described here should be applicable to the study of a variety of aspects of recombination in virt

ALERT

Intrachromosomal Homologous Recombination in , into the mutant background, how recombination frequencies are determined, and, if necessary, how cells carrying a restored .A gene can be isolated and propagated for molecular analysis of the particular recombination event.

脫毛

acetylcholine

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