Titlebook: Genetic Engineering; Principles and Metho Jane K. Setlow Book 1987 Plenum Press, New York 1987 genetic engineering.Tree Biology

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A New Method for Synthesizing RNA on Silica Supports,ld be synthesized using either the SP6 or T7 promoter and an appropriate DNA duplex (1,2). However, several recent developments primarily centered on studies of intron splicing mechanisms (3–6) have led us to initiate a program in this area. We now feel that eventually new chemical methods for synth

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Activity Gels: Reformation of Functional Proteins in SDS-Polyacrylamide Gels,ermining the subunit structures of purified proteins (1). The two-dimensional gel system developed by O’Farrell, that combines isoelectric focusing in urea with SDS-PAGE, offers the additional advantage of greater resolution than SDS-PAGE alone (2,3). Even small amounts of proteins can be resolved a

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High Level Production of Proteins in Mammalian Cells,ided a valuable aid to the understanding of the DNA sequence requirements for gene expression. In addition, this technology has provided a means to understand protein structure and function, by enabling the production of large amounts of proteins that previously could be isolated in only minute quan

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Alternative Splicing: Mechanistic and Biological Implications of Generating Multiple Proteins from These specific cell phenotypes result from the regulated expression of protein isoforms that are structurally distinct, developmentally regulated and cell specific. The molecular mechanisms responsible for the generation of this protein diversity fall into two main classes: those that select a part