Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

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https://doi.org/10.1007/978-0-85729-856-0polymerase chain reaction (PCR)-based methods. By incorporating a RNA promoter element during reverse transcription of messenger RNA (mRNA), a cDNA library can be preserved and amplified in the form of antisense RNA (aRNA) construct. In brief, the aRNA amplification procedure (. .) is based on (1) r

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Transforming the Russian Economyte the fact that obtaining amounts of material suitable for direct processing by standard methods is often time-consuming and expensive and may be even impossible. Perhaps the most significant obstacle to the full appreciation of the technique is the widespread belief that polymerase chain reaction

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https://doi.org/10.1007/978-3-319-93236-1f the cells under certain special conditions, such as pathogenesis (.,.), cancer staging (.), drug treatment, and developmental processes (.). Traditionally, gene transcripts were extracted from lysed cells with pheno-chloroform followed by precipitation, and messenger RNAs (mRNA) were further purif

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Settling in with the Ojibwe at Bad River,chain reaction (RT-PCR) (.,.). There are a number of variables that must be controlled for if accurate and reproducible results are to be obtained (.). A significant limitation to the accurate quantitation of many mRNA species is genomic DNA contamination during the RNA purification step (.). This c

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Islamophobia and the Talking Heads,ingle cells (.). By incorporating a RNA promoter element during the synthesis of double-stranded complementary DNA (cDNA) templates, a poly(A.) RNA library can be generated and reamplified from the templates in the same conformation and composition as its mRNA origins (.). Using microarray analysis,

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