Titlebook: Gene Therapy Protocols; Paul D. Robbins Book 19971st edition Springer Science+Business Media New York 1997

不合

Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes,lied using protein ligands to the hepatic asialoglycoprotein receptor (ASGr) (.,.-.), and, subsequently, the transferrin receptor (.). The ASGr is a cell-surface receptor that is highly represented on hepatocytes. Thus, genes targeted to this receptor can be delivered in a highly selective manner to the liver.

虛弱的神經(jīng)

Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung,n utero gene therapy and then outline the methodology and large animal model we are currently using to consider retrovirus-mediated gene transfer to fetal lung. In this latter regard, the candidate inherited disorder is cystic fibrosis and the reader is referred to Chapters 1 and 12 in this volume.

vector

, Retrovirus-Mediated Gene Transfer into the Liver,, the need for chronic immunosuppression, and the availability of organs. Gene therapy would pro vide an alternative to transplantation by restoring the expression of the defective gene in an organ that is otherwise structurally and functionally normal.

凌辱

Methods for Gene Transfer Using DNA-Adenovirus Conjugates,sess endogenous pathways for internalization of macromolecules, the utilization of these pathways for the purpose of DNA delivery represents a strategy that potentially allows certain practical advantages. In this regard, these cellular internalization pathways can be highly efficient. For example,

光亮

Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors,ive way of delivering genes into mammalian cells. This chapter aims to explore the various practical aspects of the AAV vector system, and in consequence, to highlight particular difficulties that may be encountered by workers new to the field. However, before describing the methodology involved in