Titlebook: Gene Expression Analysis; Methods and Protocol Nalini Raghavachari,Natàlia Garcia-Reyero Book 2018 Springer Science+Business Media, LLC, pa

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https://doi.org/10.1057/9781137479655 of miRNA library for next-generation sequencing to increase the efficiency of adapter ligation and finally construct a more specific cDNA library for sequencing and analyses for miRNA expression profiling.

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RNA-Seq and Expression Arrays: Selection Guidelines for Genome-Wide Expression Profiling, analytical differences between the two platforms, reports in the literature comparing arrays and RNA-Seq for gene expression, and our own example study and experience, we provide recommendations for platform selection for gene expression studies.

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Transcript Profiling Using Long-Read Sequencing Technologies, In this chapter, we describe experimental procedures for library preparation, sequencing, and associated data analysis approaches for PacBio and ONT with a major focus on full length cDNA synthesis, de novo transcriptome assembly, and isoform quantification.

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A Review of Transcriptome Analysis in Pulmonary Vascular Diseases, in diseased onset and progression, developed gene signatures to appropriately classify types of pulmonary hypertension and severity of illness, and identified novel gene mutations leading to hereditary forms of the disease.

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Juan Albarracin-Jordan,Thérèse Bouchardh comprehensive transcriptional characterization of mRNA, miRNA, lncRNA, and small RNA in a robust and successful manner. Transcriptomic strategies are gaining momentum across diverse areas of biological, plant sciences, medical, clinical, and pharmaceutical research for biomarker discovery, and disease diagnosis and prognosis.

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