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Titlebook: Gene Essentiality; Methods and Protocol Long Jason Lu Book 2015 Springer Science+Business Media New York 2015 Candida albicans.Computationa

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書(shū)目名稱(chēng)Gene Essentiality
副標(biāo)題Methods and Protocol
編輯Long Jason Lu
視頻videohttp://file.papertrans.cn/382/381915/381915.mp4
概述Includes cutting-edge methods and protocols for the study of microbial gene essentiality.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice fr
叢書(shū)名稱(chēng)Methods in Molecular Biology
圖書(shū)封面Titlebook: Gene Essentiality; Methods and Protocol Long Jason Lu Book 2015 Springer Science+Business Media New York 2015 Candida albicans.Computationa
描述.This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and .Candida albicans.. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful .Methods in Molecular Biology. series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and up-to-date, .Gene Essentiality: Methods and Protocols. will aid researchers who wish to further our knowledge in this vital field of study..
出版日期Book 2015
關(guān)鍵詞Candida albicans; Computational predictions; Microbial essential genes; Prokaryotes; Single-gene knockou
版次1
doihttps://doi.org/10.1007/978-1-4939-2398-4
isbn_softcover978-1-4939-4862-8
isbn_ebook978-1-4939-2398-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
The information of publication is updating

書(shū)目名稱(chēng)Gene Essentiality影響因子(影響力)




書(shū)目名稱(chēng)Gene Essentiality影響因子(影響力)學(xué)科排名




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Identifying Essential , Genes Using Genome-Wide Deletion Mutation,enes in the . SK36 strain using genome-wide deletion mutation. A fusion PCR-based method is used to construct gene deletion fragments, which contain kanamycin resistance cassettes with two flanking arms of DNA upstream and downstream of the target gene. The linear fused PCR amplicons were transforme
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Identifying Essential Genes in , by Global Phenotypic Profiling,libraries after pressure from selection. Although this method can be used for any organism in which transposon mutagenesis is possible, this chapter describes its use in .. More specifically, the methods for generating complex libraries through transposon mutagenesis, design of selective pressure, e
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Essential Genes in the Infection Model of ,-PCR-Based Signature-Tagged Mutagenesis,tion of genes that will diminish or enhance maintenance of an organism in a specific ecological niche or environment. PCR-based STM applied to . permitted the identification of genes essential for in vivo maintenance by transposon insertion and negative selection in a mixed population of bacterial m
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A Statistical Framework for Improving Genomic Annotations of Transposon Mutagenesis (TM) Assigned Eiment to identify essential genes in Prokaryota. However, due to the limitation of high-throughput technique, this approach yields substantial systematic biases resulting in the incorrect assignments of many essential genes. To obtain unbiased and accurate annotations of essential genes from TM expe
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