Titlebook: G Protein-Coupled Receptor Screening Assays; Methods and Protocol Duarte Miguel F. Prazeres,Sofia Aires M. Martins Book 2015 Springer Scien

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Ca2+ Mobilization Assays in GPCR Drug Discoveryfluorescence-based method (FLIPR Calcium 4 Assay) developed by Molecular Devices for a FlexStation and routinely used in our laboratory for detecting intracellular calcium changes. The assay is designed to study calcium mobilization induced by majority of GPCRs and calcium channels and allows for si

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Monitoring G Protein-Coupled Receptor Activation Using the Protein Fragment Complementation Techniqu, proliferation, hormonal regulation, and neuronal activity. Further, they are involved in many disease-relevant processes, such as cancer and neurodevelopmental diseases, and represent the largest class of drug targets. Therefore, monitoring how GPCRs are regulated in their activity is crucial to u

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Use of ImageJ to Recover Information from Individual Cells in a G Protein-Coupled Receptor Assay to accurately and timely extract useful information from image and video data has become more and more important. Image J is an open-source program that provides powerful tools with a simple interface designed to fit the needs of image analysis of most researchers. In this chapter, Image J routines

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