Titlebook: Epitranscriptomics; Methods and Protocol Narendra Wajapeyee,Romi Gupta Book 2019 Springer Science+Business Media, LLC, part of Springer Nat

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,Synthesis and Evaluation of Novel Neamine–Nucleoside Conjugates as Potential Antibiotic Targets forNeamine–nucleoside conjugates could bind to the groove of RNA while the nucleobase moiety would bind specifically to the sequence of the 16S rRNA A-site fragment. Thus the designed conjugate compound . was found to have the same dissociation constant as neamine for binding to 16S rRNA and the neamin

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LncVar: Deciphering Genetic Variations Associated with Long Noncoding Genes,ctions of most lncRNAs remain to be explored. Previous studies have revealed that a large amount of disease-associated variations are located in the lncRNA gene regions. To evaluate the effects of genetic variations on lncRNAs, we constructed a database of genetic variations associated with long non

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Guided Reconstruction of Full-Length Isoforms from Short Reads by CIDANE,logies (RNA-seq) provide an immediate measurement of local splicing events, the phasing of these events along full-length isoforms requires the computational inference of long-range dependencies from short-range data points. We introduce CIDANE, a tool for the assembly and quantification of full-len

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5-Methylcytosine Analysis by RNA-BisSeq,wn or novel m.C sites have been validated by using advanced high-throughput techniques combined with next-generation sequencing (NGS), especially RNA bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of

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