Titlebook: Epigenetic Methods in Neuroscience Research; Nina Karpova Book 2016 Springer Science+Business Media New York 2016 ChIP.DNA.chromatin immun

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https://doi.org/10.1007/978-3-662-44420-7itro studies have demonstrated that dendritically synthesized BDNF, derived from long 3′ UTR . mRNA, is required for spine maturation and spine pruning. In this chapter, we describe methodology used to understand the molecular mechanisms through which dendritically synthesized proteins (i.e., BDNF) modulate synaptic plasticity.

Temporal-Lobe

https://doi.org/10.1007/978-3-531-93301-6NA) probes and the tyramide signal amplification (TSA) system for detection of microRNAs in frozen brain tissue sections. Combining this miRNA-FISH method with immunofluorescence using neuron-specific antibodies allows cell type-specific localization of miRNAs in the brain.

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Tractable

heart-murmur

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