Titlebook: Environmental Microbiology; Methods and Protocol Ian T. Paulsen,Andrew J. Holmes Book 2014Latest edition Springer Science+Business Media, L

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https://doi.org/10.1007/978-3-030-50049-8 such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representat

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The Golden Age of the Swedish Sport Model,ation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative P

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A Political History of the Two Irelandssity can be generated in a sensitive, reproducible, and cost-effective manner. This protocol describes the steps required to generate T-RFLP profiles of the dominant members of a bacterial community, by PCR amplification of the bacterial 16S rRNA genes and three restriction endonuclease digests to g

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Susan K. Foley,Charles Sowerwineial. We describe a real-time quantitative PCR (qPCR) method that targets a genetic marker of the human-associated . for identification of human fecal pollution in ambient water samples. The following protocol includes water sample collection, filtration, DNA isolation with a sample processing contro

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Conclusion: For Unto Us Post-truth Is Born,ant carbon source. The development and application of a microarray targeting the particulate methane monooxygenase gene (.) have allowed a high-throughput, semiquantitative analysis of the major methanotroph groups in a number of different environments..Here we describe the use of a .-based short ol

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Assessing the theoretical approach,imetric reaction that is indicative of respiration, these microplate assays measure the response of an individual strain or microbial community to a large and diverse range of nutrients and chemicals. Phenotype MicroArrays have been used to study gene function and to improve genome annotation in sin

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https://doi.org/10.1007/978-1-349-08277-3nctions as a metabolic “fingerprint” of an individual cell, which enables differentiation of cell types, physiological states, nutrient condition, and variable phenotypes. Raman tweezers combines single-cell Raman spectroscopy with optical laser tweezers to allow the identification and isolation of

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