Titlebook: Engineering Natural Product Biosynthesis; Methods and Protocol Elizabeth Skellam Book 2022 The Editor(s) (if applicable) and The Author(s),

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Elizabeth SkellamIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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collagen

A Bioinformatics Workflow for Investigating Fungal Biosynthetic Gene Clusters, of such predictions remains restricted as they provide total numbers of biosynthetic pathways with only very limited biological significance. In this chapter, we describe a workflow to predict and analyze biosynthetic gene clusters in fungal genomes. It relies on similarity networking and phylogeny

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phase-2-enzyme

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Heterologous Expression of Fungal Biosynthetic Pathways in , Using Episomal Vectors, are often found in fungal species that are genetically intractable or difficult to cultivate, heterologous expression is increasingly being used for compound discovery. In addition, heterologous expression is a useful strategy to elucidate the function of the genes within a BGC and shed light on th

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擴(kuò)張

Investigating Fungal Biosynthetic Pathways Using , as a Heterologous Host,industries. Recently, heterologous expression has become an irreplaceable technique to functionalize fungal biosynthetic gene clusters and synthesize fungal natural products in various chassis organisms. This chapter describes the general method of using . as a chassis host to investigate fungal bio

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