Titlebook: Embryo Models In Vitro; Methods and Protocol Magdalena Zernicka-Goetz,Kursad Turksen Book 2024 The Editor(s) (if applicable) and The Author

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Stem Cell-Derived Microfluidic Amniotic Sac Embryoid (,PASE),we provide detailed instructions needed to reproduce μPASEs, including the immunofluorescence staining and cell retrieval protocols for characterizing μPASEs obtained under different experimental conditions.

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Guinea Pig Preimplantation Embryos: Generation, Collection, and Immunofluorescence,flush and smear to confirm successful mating, performing euthanasia of the guinea pig, and flushing in vivo embryos. Once collected, the embryos can be utilized for numerous downstream applications. Here we will cover embryo culturing and processing embryos for immunofluorescence.

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Book 2024in vitro phenocopying many of the developmental landmarks and 3D architectures seen normally in vivo. The protocols gathered herein cover diverse aspects of the topic and exemplify some of the key developments and improvements in the field. Written for the highly successful .Methods in Molecular Bio

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978-1-0716-3688-6The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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stoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.

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