Titlebook: ERK Signaling; Methods and Protocol Gerardo Jimenez Book 2017 Springer Science+Business Media New York 2017 signaling pathways.ERK2 protein

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Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA),ties in RAS/MAPK signaling cascade have been intimately implicated in numerous human diseases, including cancer. Herein, we describe a novel methodology to study activation of this pivotal signaling pathway. The Proximity Ligation Assay (PLA) is employed to monitor kinase–substrate interactions betw

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Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors,lls with subcellular resolution. There are quite a number of already existing sensors and this technology is increasingly used to obtain quantitative dynamic datasets. In this chapter, we describe the analysis of endogenous extracellular signal-regulated kinase (ERK) activity in living cells using t

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Co-culture Activation of MAP Kinase in , S2 Cells,ological detection of phosphorylated MAPK can be used to monitor signaling in vivo, identify novel pathway components, and assess ligand activity. In this chapter, I describe a cell co-culture method to assess activity of cell-bound extracellular ligands that result in phosphorylation of the ERK (ex

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978-1-4939-8195-3Springer Science+Business Media New York 2017

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Gerardo JimenezIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains keynotes and implementation advice from the experts