Titlebook: Drosophila Oogenesis; Methods and Protocol Michelle S. Giedt,Tina L. Tootle Book 2023 The Editor(s) (if applicable) and The Author(s), unde

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,,ncertain. Of the cytoskeletal proteins, the activity and biological significance of nuclear actin has been the most extensively researched. It is now clear that actin performs essential tasks both in the cytoplasm and the nucleus, and that the dynamic balance between the large cytoplasmic and the si

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Methods in Molecular Biologyhttp://image.papertrans.cn/e/image/282978.jpg

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Exploiting the United Kingdom’s Oil Reservesope. We further outline steps to quantify various cellular behaviors and protein dynamics of live migrating border cells using the Fiji image processing package of ImageJ. These methods can be adapted to other migrating cell collectives in cultured tissues and organs.

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Housing,t ovaries, apply a fluorescent LD dye, and culture follicles such that they remain alive and healthy during imaging. Finally, a method is provided that employs in vivo centrifugation to assess colocalization of markers with LDs.

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Quantitative Image Analysis of Dynamic Cell Behaviors During Border Cell Migration,ope. We further outline steps to quantify various cellular behaviors and protein dynamics of live migrating border cells using the Fiji image processing package of ImageJ. These methods can be adapted to other migrating cell collectives in cultured tissues and organs.

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1064-3745 expertsThis detailed volume compiles numerous methods to explore fly oogenesis. Beginning with updated protocols from isolating and staining the ovary to numerous imaging techniques and genetic protocols for cell-specific assessment and CRISPR-mediated mutagenesis, the volume continues by detailing

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