Titlebook: Deadenylation; Methods and Protocol Eugene Valkov,Aaron C. Goldstrohm Book 2024 This is a U.S. government work and not under copyright prot

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Gonzalo L. Villarreal,Verónica Artolaics. However, direct RNA sequencing using the Oxford Nanopore Technologies (ONT) platform makes it possible to conduct transcriptome-wide analyses at the single-molecule level without the PCR bias introduced by other methods. In this chapter, we provide a protocol to measure both RNA levels and poly(A)-tail lengths in the yeast . using ONT.

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https://doi.org/10.1007/978-3-031-58799-3 shortening of these poly(A) tails by deadenylase enzymes has a critical role in post-transcriptional gene regulation. However, deadenylases are usually large, multisubunit, and multifunctional complexes, which complicates their biochemical analysis. This chapter presents a methodology for isolating

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Active, Not Recruiting Studies,osine (poly(A)) RNA tract, thereby providing insight into the mechanism of deadenylation. Importantly, this assay can be performed in both homogeneous and heterogeneous environments without relying on gel electrophoresis of RNA products or coupled enzymatic reactions that indirectly report on the RN

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