Titlebook: DNA-Protein Interactions; Principles and Proto Beno?t P. Leblanc,Sébastien Rodrigue Book 2015Latest edition Springer Science+Business Media

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Probing of Nascent Riboswitch Transcripts,lecules that are involved in the biosynthesis and transport of cellular metabolites. Because riboswitches regulate gene expression by modulating their structure, it is vital to employ native probing assays to determine how native riboswitch structures perform highly efficient and specific ligand rec

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Functional Studies of DNA-Protein Interactions Using FRET Techniques, and repair. Identifying and examining the nature of these interactions is therefore a crucial prerequisite to understand the molecular basis of how these fundamental processes take place. The application of fluorescence techniques and in particular fluorescence resonance energy transfer (FRET) to p

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,Precise Identification of Genome-Wide Transcription Start Sites in Bacteria by 5′-Rapid Amplificatirough 5′-rapid amplification of cDNA ends (5′-RACE). This technique has now been adapted for high-throughput sequencing and can be used to precisely identify TSS in a genome-wide fashion for practically any bacterium, which greatly contributes to our understanding of gene regulatory networks in micr

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Detection of Short-Range DNA Interactions in Mammalian Cells Using High-Resolution Circular Chromoscial to understanding the molecular mechanisms that regulate gene expression. In this chapter, we present a protocol for high-resolution circular chromosome conformation capture coupled to deep sequencing. This methodology allows to investigate short-range DNA interactions (<100 kbp) and to obtain h