Titlebook: DNA Topoisomerase Protocols; Volume II: Enzymolog Neil Osheroff,Mary-Ann Bjornsti Book 2001 Humana Press 2001

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Book 2001ze changes in the linkage of DNA strands. DNA topoisomerases are ubiquitous in nature and have been shown to play critical roles in most p- cesses involving DNA, including DNA replication, transcription, and rec- bination. These enzymes further constitute the cellular targets of a number of clinical

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https://doi.org/10.1007/978-3-031-14489-9gyrase function specifically by inhibiting ATP hydrolysis (.–.). In addition, several anticancer drugs that act by enhancing topoisomerase II-mediated DNA cleavage also impair interactions between the enzyme and ATP (.).

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Xuecheng Hou,Ning Kang,Yixiang Chenhe filter, or found in filtrates and washes. Because protein-adsorbing materials can differ in their efficiency, capacity, and flow characteristics, filters from the same lot should be used in serial experiments, and the optimal filtration conditions need to be empirically determined in each case.

Geyser

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,Filter Binding Assays for Topoisomerase—DNA Complexes,he filter, or found in filtrates and washes. Because protein-adsorbing materials can differ in their efficiency, capacity, and flow characteristics, filters from the same lot should be used in serial experiments, and the optimal filtration conditions need to be empirically determined in each case.

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Habiba Drias,Farouk Yalaoui,Allel Hadjalihe directional crossing of a DNA duplex through a transient enzyme-bridged double-strand break in a 120–150 bp segment of DNA wrapped on the enzyme (.–.). This process changes the linking number of DNA in steps of two, and together with an ability to form and resolve DNA knots and catenanes, establishes gyrase as a type II topoisomerase.

植物茂盛

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Plasmid DNA Supercoiling by DNA Gyrase,he directional crossing of a DNA duplex through a transient enzyme-bridged double-strand break in a 120–150 bp segment of DNA wrapped on the enzyme (.–.). This process changes the linking number of DNA in steps of two, and together with an ability to form and resolve DNA knots and catenanes, establishes gyrase as a type II topoisomerase.

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