Titlebook: DNA Recombination; Methods and Protocol Hideo Tsubouchi Book 2011 Springer Science+Business Media, LLC 2011 DNA double-strand break.DNA mic

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Detection of RNA-Templated Double-Strand Break Repair in Yeastg through a reverse transcript intermediate. As initially demonstrated in the yeast . via transformation by RNA-containing oligonucleotides (oligos), RNA sequences can serve as templates for chromosomal double-strand break (DSB) repair. Synthetic oligos containing embedded RNA tracts of various size

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Characterization of Meiotic Crossovers in Pollen from ,anistically important for the regular segregation of homologous chromosomes. They generate either crossovers, which are reciprocal exchanges between chromosome segments, or gene conversions. Both kinds of events occur in narrow regions (less than 10?kb) called hotspots, which are distributed along c

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Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40omologous DNA fragment by HR is usually a very rare event in vertebrate cells; however, in chicken B lymphoma cell line DT40, the ratio of targeted to random integration is extremely high. Although the underlying mechanism of this phenotype is not fully understood, DT40 has been utilized as a model

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https://doi.org/10.1007/978-1-137-40757-3log, for kinase assays that detect only those proteins that are directly phosphorylated by Mek1. This method may be applicable to any kinase for which an analog-sensitive version is available. In addition, it provides a non-radioactive alternative for kinase assays with wild-type kinases.