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Titlebook: Cytochrome P450 Protocols; Ian R. Phillips,Elizabeth A. Shephard Book 2006Latest edition Humana Press 2006 Allele.Escherichia coli.Oxidati

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樓主: metamorphose
21#
發(fā)表于 2025-3-25 06:11:33 | 只看該作者
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發(fā)表于 2025-3-25 13:09:03 | 只看該作者
Komplexverbindungen Bindungen in Komplexenonitor P450 (P450) activity in both hepatic and extrahepatic tissues. A spectrofluorometric method is described for determination of P450-catalyzed 7-ethoxycoumarin .-deethylation. Following acidification of the incubation mixture, the enzymatic product, 7-hydroxycoumarin, is recovered by a double-e
24#
發(fā)表于 2025-3-25 17:05:10 | 只看該作者
Cytochrome P450 Protocols978-1-59259-998-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
25#
發(fā)表于 2025-3-25 22:44:11 | 只看該作者
Hans Peter Latscha,Helmut Alfons Klein microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.
26#
發(fā)表于 2025-3-26 03:22:33 | 只看該作者
,Determination of CYP2C9-Catalyzed Diclofenac 4′-Hydroxylation by High-Performance Liquid Chromatogr microsomes or cDNA-expressed CYP2C9. Analytical separation is achieved using a C. column developed with a gradient of 30% acetonitrile and 2 m. perchloric acid in water to 100% methanol, with detection at 280 nm. This method is applicable to enzymatic studies for determination of CYP2C9-catalyzed diclofenac 4′-hydroxylation activity.
27#
發(fā)表于 2025-3-26 05:49:44 | 只看該作者
28#
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29#
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發(fā)表于 2025-3-26 17:15:25 | 只看該作者
Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System,bolizing enzymes toward a particular substrate. In this chapter we describe a baculovirus/insect cell system that we have used for the expression of human and mouse flavin-containing monooxygenases. Methods are described for the generation of recombinant baculoviral DNAs, via both site-specific tran
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