Titlebook: Cancer Cell Dormancy; Methods and Protocol Marco Montagner Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive licen

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Preclinical Mouse Intraductal Model (MIND) to Study Metastatic Dormancy in Estrogen Receptor-Positithe use of GFP/RFP:Luc2 reporters, we can monitor in vivo tumour growth and conduct ex vivo metastases assays to evaluate dormant metastatic cell harboring organs. Upon recovery of metastatic cells from ER. breast cancer models, downstream analyses can be conducted to assess the relationship between epithelial plasticity and metastatic dormancy.

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1064-3745 tial for reproducible results.This volume provides protocols and techniques to study metastatic dormant cells. Chapters focus on in vitro systems to model metastatic dormancy in vitro, detection of disseminated tumor cells in vivo, perturbation of key molecules involved in metastatic dormancy, ?and

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In Ovo Xenograft Model to Study Tumor Dormancy, Tumor Cell Dissemination and Metastasis,ntages of this system are its high reproducibility, cost-effectiveness, and versatility. Here, by using two dormancy tumor models, one of head and neck squamous cell carcinoma and one of breast cancer, we described a detailed protocol for the use of the CAM model in metastasis assays and for the study of tumor growth and dormancy.

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13C Tracer Analysis and Metabolomics in Dormant Cancer Cells,ronment. Thus, studying the metabolic features of dormant disseminated tumor cells has gained importance in understanding the dormancy process. Here, we describe a method to perform metabolomics and .C tracer analysis in 3D cultures of dormant breast cancer cells.

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In Vitro and In Vivo Systems to Study Tumor Dormancy and the Transition to Overt Metastases Inducedr even decades after the initial treatment of the primary tumor. This latency in the manifestation of the disease is attributed to the presence of early disseminated tumor cells (DTCs) that lay quiescent (dormant) for years until they emerge as clinically overt metastases. Given that to date we have

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Modeling Tumor Cell Dormancy in an Ex Vivo Liver Metastatic Niche, aid in developing effective therapies to either maintain or eliminate these dormant cells and thus prevent them from emerging into overt metastatic disease. To study the behavior of dormant tumor cells—mechanisms that promote, maintain, and disrupt this state—we utilize the Legacy LiverChip?, an al