Titlebook: Cryptococcus neoformans; Methods and Protocol Erin E. McClelland Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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Yanfeng Zhang,Qiange Wang,Shufeng Gongand life-threatening infections of the central nervous system. Following inhalation of yeasts or desiccated basidiospores into the lung alveoli, resident pulmonary phagocytic cells aid in the identification and eradication of . yeast through their arsenal of pattern recognition receptors (PRRs). PRR

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Chunming Yang,Xinghao Song,Hui Zhang,Bo Li such, measuring fungal burden of . strains—and indeed how drug treatments can influence fungal burden—provides important information about . pathogenesis. In this chapter, we describe two methods that may be used to appraise fungal burden: a standard end-point colony-formation assay for calculating

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Erin E. McClellandIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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Cryptococcus neoformans978-1-0716-3722-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-981-16-0479-9s of the data is typically a multistep process using several bioinformatics tools. The following protocol utilizes a reliable pipeline for identifying differentially expressed genes among samples of . that is approachable for the adventurous beginner.

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