Titlebook: Cryo-Electron Tomography; Structural Biology i Friedrich F?rster,Ariane Briegel Book 2024 The Editor(s) (if applicable) and The Author(s),

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Shauna Butterwick,Marilou Carrillomolecular complexes. However, extracting this information is particularly challenging for complexes that cannot be identified visually and are embedded in cellular environments comprising multitude of macromolecular?species that lack an obvious ordering pattern. To overcome these challenges, a numbe

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A Mother/Daughter Distance Dancenly sharing such data enables validation of research findings, sparks further biological discoveries, and fuels development of improved software tools for data processing. Data sharing is complicated, however, by the size of cryo-ET datasets. Here we synthesize our experience archiving and sharing n

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Friedrich F?rster,Ariane BriegelComprehensive description of cryo-electron tomography.Addresses the underlying principles and all recent developments of cryo-ET.A valuable resource for scientists working in the field of structural b

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Principles of Tomographic Reconstruction,sive imaging method, two-dimensional (2D) images of slices, or sections, within an organism are analyzed to determine the three-dimensional (3D) localization of internal objects. These sections can be so thin that the target objects are unobscured by the superposition of over- or underlying structur

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In Situ Cryo-Electron Tomography and Advanced Micromanipulator Techniques,ed structures is rapidly expanding and requires that preparation techniques evolve to keep up with the growing demand for larger specimens. In particular, organoids and tissue, but also small animals that can be vitrified in whole by high-pressure freezing, are targets of this new branch of in situ