Titlebook: Crop Breeding; Methods and Protocol Delphine Fleury,Ryan Whitford Book 2014 Springer Science+Business Media New York 2014 crop improvement.

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Defining a Genetic Ideotype for Crop Improvement,r provides a road map for the establishment of a refined genetic ideotype. The first step is an accurate definition of the target environment including the underlying constraints, their probability of occurrence, and impact on phenotype. Once the environmental constraints are established, the wealth

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Temperature Switch PCR (TSP): A Gel-Based Molecular Marker Technique for Investigating Single Nucleudies of various organisms. The technique consists of a biphasic PCR with two sets of primers, a locus-specific set and a nested locus-specific set. The PCR products can be easily assessed for polymorphism based on different band sizes using agarose gel electrophoresis.

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