Titlebook: Clathrin-Mediated Endocytosis; Methods and Protocol Laura E.‘Swan Book 2018 Springer Science+Business Media, LLC, part of Springer Nature 2

adhesive

https://doi.org/10.1007/978-1-4613-9407-5cribe a methodology to follow adaptor-induced clathrin assembly in real-time using fluorescence microscopy on a facile model membrane assay system of .upported .emb.ane .ubes (SMrT). Results from such assays can be conveniently run through routine image analysis procedures to extract kinetic parameters of the clathrin assembly reaction.

Conspiracy

Generalize

共同時代

SMrT Assay for Real-Time Visualization and Analysis of Clathrin Assembly Reactions,cribe a methodology to follow adaptor-induced clathrin assembly in real-time using fluorescence microscopy on a facile model membrane assay system of .upported .emb.ane .ubes (SMrT). Results from such assays can be conveniently run through routine image analysis procedures to extract kinetic parameters of the clathrin assembly reaction.

mastoid-bone

Francis A. Gunther,Jane Davies Gunthered by mRNA interference or dominant-negative mutant overexpression affect endocytosis long before cells are being assayed. Here, we describe simple experimental procedures to assay endocytosis along the cell cycle with minimal perturbations.

Hypopnea

Glenn C. Miller,Richard G. Zepp,5)P. spatial distribution, and semi-quantification of PI(4,5)P. levels in the plasma membrane using fluorescence microscopy. Depending on the probe selected for lipid detection, this simple assay can be modified to study other plasmalemmal phospholipids and/or proteins.

整潔

Libido

Spatial and Temporal Aspects of Phosphoinositides in Endocytosis Studied in the Isolated Plasma Mem,5)P. spatial distribution, and semi-quantification of PI(4,5)P. levels in the plasma membrane using fluorescence microscopy. Depending on the probe selected for lipid detection, this simple assay can be modified to study other plasmalemmal phospholipids and/or proteins.

飛鏢

Annette E. McCann,D. Roy Cullimored in our system. In this chapter, we describe in detail how to use the hot-wiring system to trigger endocytosis in human cell lines and how to image the vesicles that form using microscopy and finally, how to analyze those images.

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