Titlebook: Citrus Tristeza Virus; Methods and Protocol Antonino F. Catara,Moshe Bar-Joseph,Grazia Licciar Book 2019 Springer Science+Business Media, L

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Is Software Engineering , Engineering?ure can be performed in automated sequencers equipped with an appropriate capillary (CE-SSCP), which increases the potential of high-throughput analysis, precision, and the reproducibility of results. CE-SSCP can be also directly applied to the virus particles obtained by elution from ELISA plates or tissue-print membranes.

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Takehisa Onisawa,Janusz Kacprzykniques used in previous studies yield very limited information about the strain composition of populations and the relative titer of their components. In this chapter we describe a protocol for the characterization of CTV populations, based on the use of the next-generation sequencing Illumina MiSeq platform of p33 gene amplicons.

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A Short Note on Reflections and Publications on , (CTV) Methodologies,hybridization probes, the genetic diversity among local CTV isolates was demonstrated. With the emergence of the PCR technology, we developed a CTV-dsRNA cloning method based on the ligation of known oligonucleotide molecules to dsRNA ends and the use of complementary oligonucleotides for cDNA synth

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CTV Vectors and Interactions with the Virus and Host Plants,?h for each. CTV infection is readily determined by serology using a polyclonal antiserum or a monoclonal antiserum cocktail; whereas, molecular genotyping is conducted with reverse transcription polymerase chain (RT-PCR) or real time quantitavtive RT-PCR (RT-qPCR) with strain-specific primers and p

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