Titlebook: Circular RNAs; Christoph Dieterich,Marie-Laure Baudet Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclu

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Circular RNAs978-1-0716-3678-7Series ISSN 1064-3745 Series E-ISSN 1940-6029

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https://doi.org/10.1007/978-1-349-08714-3ar counterparts. This is particularly challenging as the back-splice junction is the only sequence not shared between the linear and circular version. In this chapter, we describe a method to study circRNA function in vivo targeting shRNAs against the desired back-splice junction to achieve knockdown with tissue-specific resolution in flies.

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https://doi.org/10.1007/978-3-030-43527-1ilico from RNA sequencing data using the . circRNA analytics software suite. The protocol starts from raw sequencing data with circRNA detection via back-splice events and includes statistical testing of circRNAs as well as primer design for follow-up wet lab experiments.

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https://doi.org/10.1007/978-981-33-6381-6l PCR (ddPCR) is rapidly taking over for the more popular qPCR. In this chapter, we describe the detailed procedure based on droplets partitioning to quantify both linear and circRNA abundancy and demonstrate the circularity of the transcript under study with high precision, in a single experiment.