Titlebook: Cilia; Methods and Protocol Peter Satir,S?ren Tvorup Christensen Book 2016 Springer Science+Business Media New York 2016 intraflagellar tra

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STED and STORM Superresolution Imaging of Primary Cilia,tudies of ciliary proteins. Superresolution microscopy can reach up to a 20 nm resolution, significantly improving the ability to map molecules in primary cilia. Here we describe detailed experimental procedure of STED microscopy imaging and dSTORM imaging, two of the most powerful superresolution i

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Methods for Visualization of Neuronal Cilia,es in neuronal specification, maintenance, and function. In mammals, the most common cilia marker, acetylated α-tubulin, is extremely difficult to detect in neuronal cilia. Here, we describe methods to detect neuronal cilia in culture, in fixed sections, and in vivo, taking advantage of transgenic m

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Analysis of Axonemal Assembly During Ciliary Regeneration in ,,. to analyze the sequence in which ciliary proteins assemble. In addition, the methods described allow analysis of the mechanisms involved in regulation of ciliary length, the proteins required for ciliary assembly, and the temporal expression of genes encoding ciliary proteins. Ultimately, these me

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, as a Model System for the Analysis of Ciliary Assembly and Motility,lia are exposed to the extracellular environment. These beat in a synchronized manner against secreted mucus thereby propelling the animal forward. Similar to the nematode ., the planarian . is easy to maintain in the laboratory and is highly amenable to simple RNAi approaches through feeding with d

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Dimensionen informellen Regierenses in neuronal specification, maintenance, and function. In mammals, the most common cilia marker, acetylated α-tubulin, is extremely difficult to detect in neuronal cilia. Here, we describe methods to detect neuronal cilia in culture, in fixed sections, and in vivo, taking advantage of transgenic mice carrying fluorescently tagged cilia proteins.

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