Titlebook: Chromosomal Mutagenesis; Shondra M. Pruett-Miller Book 2015Latest edition Springer Science+Business Media New York 2015 Genome editing too

Paradox

Donor Plasmid Design for Codon and Single Base Genome Editing Using Zinc Finger Nucleases, virtually any location in the human genome within 50 bp of a desired site. Such high resolution ZFN engineering is well within the conversion tract limitations demarcated by the mammalian DNA repair machinery, resulting in a nearly universal ability to create point mutations throughout the human ge

滋養(yǎng)

Endogenous Gene Tagging with Fluorescent Proteins,ces in the desired locations in the genome of a cell can allow monitoring of endogenous activities of disease related genes. Native gene expression and regulation is preserved in these knock-in cells in contrast to cell lines with target overexpression under an exogenous promoter as in the case of t

搏斗

Silencing Long Noncoding RNAs with Genome-Editing Tools,anscriptome. However, detailed functional analysis lags behind their rapid discovery. This might be partially due to the lack of loss-of-function approaches that efficiently reduce the expression of these transcripts. Here, I describe a method that allows a specific and efficient targeting of the hi

Indict

mediocrity

百靈鳥

創(chuàng)新

Using Engineered Endonucleases to Create Knockout and Knockin Zebrafish Models,argeted knockouts through the use of zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR associated system (CRISPR/Cas). Furthermore, using the high-efficiency TALEN system, we were able to create

偽證

Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection, engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without

rheumatism

焦慮

1064-3745 ible results.Contains key notes and implementation advice fr.This new edition explores current and emerging mutagenesis methods focusing specifically on mammalian systems and commonly used model organisms through comprehensive coverage and detailed protocols. Since the first edition, major advances