Titlebook: Chromatin Accessibility; Methods and Protocol Georgi K. Marinov,William J. Greenleaf Book 2023 The Editor(s) (if applicable) and The Author

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Simultaneous Measurement of DNA Methylation and Nucleosome Occupancy in Single Cells Using scNOMe-Send nucleosome occupancy (i.e., chromatin accessibility) in single cells. scNOMe-seq combines the activity of a GpC Methyltransferase, an enzyme which methylates cytosines in GpC dinucleotides, with bisulfite conversion, whereby unmethylated cytosines are converted into thymines. Because GpC Methyltr

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Massively Parallel Profiling of Accessible Chromatin and Proteins with ASAP-SeqA, additional measurements are required to characterize the multifaceted nature of eukaryotic cells. Here, we delineate the ATAC with Select Antigen Profiling by sequencing (ASAP-seq) protocol, a scalable approach to quantifying proteins via oligo-tagged antibodies alongside accessible DNA in thousa

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1064-3745 tocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and up-to-date, .Chromatin Accessibility: Methods and Protocols. serves as an 978-1-0716-2901-7978-1-0716-2899-7Series ISSN 1064-3745 Series E-ISSN 1940-6029