Titlebook: Chromaffin Cells; Methods and Protocol Ricardo Borges Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

chapel

https://doi.org/10.1057/9780230339378xidized two electrons (e.) that are recorded as an electrical current. Amperometry is an easy-to-use and noninvasive technique for cells (unlike patch-clamp techniques for measuring membrane capacitance) and has been widely used to monitor online catecholamine release from perifused bovine chromaffin cell populations.

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https://doi.org/10.1007/978-1-349-27445-1 granules, or stress in the endoplasmic reticulum. In this chapter, we describe current methods to study mitochondrial, endoplasmic reticulum, and secretory vesicle calcium homeostasis in living chromaffin cells using engineered targeted aequorins.

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Liberalism and the Challenge of Pluralismpacitance recordings. Precise stimulation of exocytosis is achieved by local pressure application or voltage-clamp depolarizations. While the chapter is focused on insulin-secreting cells as an accessible and disease-relevant model system, the methodology is applicable to a wide variety of secretory cells including chromaffin and PC12 cells.

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Limpid

Introduction: Hoping for the Future,the steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.

反話(huà)

The Future Society and the Transition to It in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.

FEAS

Fretful

Transmission Electron Microscopy: A Method for Studying the Adrenal Chromaffin Cellsthe steps necessary to process the adrenochromaffin tissue for the transmission electron microscopy; this protocol is suitable for any kind of adrenal tissue, and personally we used it in mammals, reptiles, and amphibians.

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