Titlebook: Chimeric RNA; Methods and Protocol Hui Li,Justin Elfman Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 Gene f

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Dosimetry of Radiolabeled Antibodies, assay to detect transcriptional read-through. Here, we describe a general RT-PCR based method to confirm the process for cis-SAGe candidates. In this method, we use PCR to amplify cDNA that is reverse transcribed from the read-through precursor mRNA. The result provides a foundation for further downstream mechanistic studies.

Arbitrary

Prediction, Characterization, and In Silico Validation of Chimeric RNAscribe the detailed steps for (1) prediction of chimeric RNAs using EricScript software, (2) characterization of chimeric RNAs to discard most probable false positive events, and (3) in silico validation of chimeric RNA to select the potential cancer-specific events.

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Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cellsenerate chimeric mRNAs through a novel intergenic .-splicing pathway. Here we describe the detailed experimental procedures to detect group II intron-generated mRNA–mRNA chimeras from total RNA extracts using stringent reverse transcription conditions along with a reverse splicing-deficient group II intron as a negative control.

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Book 2020tep, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.?.Authoritative and practical, .Chimeric RNA: Methods and Protocols. is an ideal guide to this expansive field and an inspiration to researchers seeking to further our knowledge of these remarkable features of the transcriptome..

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Identification of Chimeric RNAs Using RNA-Seq Reads and Protein–Protein Interactions of Translated Cs using RNA-seq reads. Moreover, we elaborate on the domain–domain co-occurrence method to detect alterations in chimeric protein–protein interaction (ChiPPI) networks produced by chimeric RNA that are translated to chimeric proteins.

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Detection and Measurement of Chimeric RNAs by RT-PCRn of chimeric transcripts, especially when careful consideration is applied to experimental design. In this chapter, we provide guidelines and procedures for use of RT-PCR in detection and measurement of chimeric RNA expression.

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Validation of Chimeric Fusion Peptides Using Proteomics Datacribe the detailed steps for (1) downloading and processing publicly available proteomics datasets, (2) developing fusion peptide database by performing in silico tryptic digestion of chimeric proteins, and (3) software used to identify chimeric peptides in the proteomics data.